Coding

Part:BBa_K563030

Designed by: Guo Jing   Group: iGEM11_Tianjin   (2011-10-05)


pEASY with first part of Tor2

We separate Tor2 into three parts, and the first part is called 01Tor2, including the promoter. pEASY are blunt vector provided by transgene. We link 01Tor2 with this vector for further connection.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3158
    Illegal XbaI site found at 3411
    Illegal SpeI site found at 258
    Illegal PstI site found at 3371
    Illegal PstI site found at 4561
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 258
    Illegal PstI site found at 3371
    Illegal PstI site found at 4561
    Illegal NotI site found at 3392
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2321
    Illegal BglII site found at 4348
    Illegal BamHI site found at 252
    Illegal XhoI site found at 3399
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3158
    Illegal XbaI site found at 3411
    Illegal SpeI site found at 258
    Illegal PstI site found at 3371
    Illegal PstI site found at 4561
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3158
    Illegal XbaI site found at 3411
    Illegal SpeI site found at 258
    Illegal PstI site found at 3371
    Illegal PstI site found at 4561
    Illegal NgoMIV site found at 3737
    Illegal NgoMIV site found at 5012
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5911
    Illegal BsaI.rc site found at 1768
    Illegal SapI.rc site found at 4861
    Illegal SapI.rc site found at 5071


[edit]
Categories
Parameters
n/apEASY with first part of Tor2