Coding
Part:BBa_K563030
Designed by: Guo Jing Group: iGEM11_Tianjin (2011-10-05)
pEASY with first part of Tor2
We separate Tor2 into three parts, and the first part is called 01Tor2, including the promoter. pEASY are blunt vector provided by transgene. We link 01Tor2 with this vector for further connection.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 3158
Illegal XbaI site found at 3411
Illegal SpeI site found at 258
Illegal PstI site found at 3371
Illegal PstI site found at 4561 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 258
Illegal PstI site found at 3371
Illegal PstI site found at 4561
Illegal NotI site found at 3392 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2321
Illegal BglII site found at 4348
Illegal BamHI site found at 252
Illegal XhoI site found at 3399 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 3158
Illegal XbaI site found at 3411
Illegal SpeI site found at 258
Illegal PstI site found at 3371
Illegal PstI site found at 4561 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 3158
Illegal XbaI site found at 3411
Illegal SpeI site found at 258
Illegal PstI site found at 3371
Illegal PstI site found at 4561
Illegal NgoMIV site found at 3737
Illegal NgoMIV site found at 5012 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 5911
Illegal BsaI.rc site found at 1768
Illegal SapI.rc site found at 4861
Illegal SapI.rc site found at 5071
[edit]
Categories
Parameters
n/a | pEASY with first part of Tor2 |